منابع مشابه
Ribosome Synthesis in Tetrahymena Pyriformis
The cellular site of synthesis of ribosomal RNA in Tetrahymena pyriformis was studied by analyzing the purified nuclear and cytoplasmic RNA from cells pulse labeled with uridine-(3)H. The results of studies using zonal centrifugation in sucrose density gradients show that the ribosomal RNA is synthesized in the nucleus as a large precursor molecule sedimenting at 35S. The 35S molecule undergoes...
متن کاملMicronuclear Ribonucleic Acid in Tetrahymena Pyriformis
The presence of RNA in the micronucleus of Tetrahymena pyriformis was detected by electron microscope radioautography after incubation with tritiated precursors. The specificity of RNA labeling was shown by ribonuclease digestion. The period of appearance of labeled RNA in the micronucleus is approximately coincident with the DNA synthesis period for the micronucleus. Pulse-chase experiments sh...
متن کاملCiliary Membrane Differentiations in Tetrahymena Pyriformis
We have examined thin sections and replicas of freeze-fractured cilia of Tetrahymena pyriformis. The ciliary necklace located at the base of all freeze-fractured oral and somatic cilia has been studied in thin sections. Since electron-dense linkers have been found to connect both microtubule doublets and triplets to the ciliary membrane at the level of the necklace, the linkers and the associat...
متن کاملStudies of Membrane Formation in Tetrahymena Pyriformis
A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
متن کاملMacronuclear Events in Synchronously Dividing Tetrahymena Pyriformis
The macronuclei of synchronously dividing mass cultures of Tetrahymena pyriformis (strain WH(6)) were examined with the electron microscope for changes during two division cycles. Samples were prepared at 30-minute intervals for a period of 8(1/2) hours which included the time required to induce synchrony by five heat shocks (4(1/2) hours). The interphase macronucleus contains peripheral, cresc...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)95778-9